Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 16;23(4):100987. doi: 10.1016/j.isci.2020.100987

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

VHH D4 Recognizes a Surface Epitope of LSP-1 Specifically Expressed by mo-DC

(A) Expression levels (arbitrary units) of LSP1 in ascites DC; ascites macrophages; in vitro mo-DC and mo-Mac generated with M-CSF, IL-4, and TNFα; in vitro mo-DC generated with GM-CSF and IL-4; and blood CD14⁺ monocytes. Each dot represents an individual donor. Median is shown. Affymetrix data from dataset GSE102046.

(B) Normalized counts of LSP1 expression in selected human blood cells. RNA-seq data from the Human Cell Atlas (http://immunecellatlas.net).

(C and D) Monocytes were cultured with M-CSF, IL-4, and TNFα to generate DC (mo-DC) and macrophages (mo-Mac). Peripheral blood myeloid cells were also analyzed (monocytes and cDC2). Cells were stained with a commercial anti-LSP-1 antibody (C) or VHH D4 (D), with membrane permeabilization (intracellular) or not (surface). Gray shaded histograms are fluorescence-minus-one controls. Representative of five (blood) or six (in vitro-generated) individual donors.