Figure 3.

Wild-type IDH2 plays a key role in EBV-LMP1-induced tumorigenesis. A, IDH2 protein expression was detected by western blot analysis. EV, empty vector. B, cell viability was measured in empty vector- or IDH2-overexpressing cells using the MTS assay. C, viability was measured in cells transfected with scramble or IDH2 shRNA using the MTS assay. D, cell death was measured by Sytox Green staining in groups as indicated. E, tumor volume and weight were measured in xenografts in mice inoculated with HNE2-LMP1 cells transfected with scramble or IDH2 shRNA-1 (N = 5). F, photographs of xenograft tumors are shown (scale bar = 1 cm). Data are presented as means ± S.D.; ∗∗, p < 0.01; ∗∗∗, p < 0.001.