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. 2020 Feb 18;36:100966. doi: 10.1016/j.molmet.2020.02.009

Figure 5.

Figure 5

EBV-LMP1 facilitates IDH2 transcription through c-Myc. A, Myc-TA-luc and pRL-TK plasmids were co-transfected into NPC cells. The transcriptional activity of c-Myc was determined by the dual luciferase reporter assay. B, c-Myc and IDH2 protein expression was detected by western blot analysis in cells transfected with control or c-Myc siRNAs. C, IDH2 mRNA expression was measured by RT-PCR. D, ChIP analysis was used to detect the presence of c-Myc in the promoter region of IDH2 by using two primer sites. E, pGL3-IDH2 and pRL-TK plasmids were co-transfected into NPC cells. IDH2 promoter activity was determined by the dual luciferase reporter assay. F, IDH2 promoter activity was determined in cells transfected with control or c-Myc siRNAs. G, luciferase activity was determined in cells transfected with pGL3-Basic, pGL3-IDH2, and pGL3-IDH2-mut (deletion of the c-Myc-binding site from −1446 to −1437 bp in pGL3-IDH2). H, viability was measured in cells transfected with control or c-Myc siRNAs by using MTS. The levels of I, 2-HG and J, α-KG (J) were measured in NPC cells transfected with control or c-Myc siRNAs. Data are presented as means ± S.D.; ∗, p < 0.05; ∗∗, p < 0.01.