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. 2004 Dec 27;17(2):167–176. doi: 10.1093/intimm/dxh197

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© 2004 The Japanese Society for Immunology

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Fig. 4. — Vaccine-induced T cell response against an immunodominant epitope of HPV18 E7. (A) Day 91 PBMC from patient VAC002 were cultured for 7 days with HPV18 E7 pool (25 peptides) before testing in ELISPOT assays against either the whole HPV18 E7 pool or smaller HPV18 E7 pools containing four individual peptides (C1–C4 pools) or mitogens. (B) Day 91 PBMC were cultured as above, before testing against entire HPV18 E7 pool, HPV18 E7 C2 pool (peptides 45, LLCHEQLSDSEEEND; 46, LSDSEEENDEIDGVN; 47, ENDEIDGVNHQHLPA and 48, GVNHQHLPARRAEPQ) or individual HPV18 E7 peptides (45, 46, 47 or 48). The response against mitogens in experiment B exceeded 500 spots.