Table 2.

T cell responses against HPV16 and 18 peptides in healthy volunteers measured by IFN-γ ELISPOT

Donor	Spots per cultured 105 cellsa
	16 E6	16 E7	18 E6	18 E7	PPP
HV 001	—	24	44	—	≥200
HV 002	41	—	21	23	≥200
HV 003	97	—	—	—	162
HV 004	—	—	—	—	≥200
HV 005	22	—	—	—	≥200
HV 006	—	—	—	—	≥200
HV 007	22	—	103	—	≥200
HV 008	—	26	—	—	54
HV 010	34	—	—	—	71

^aPBMC from cryopreserved stocks were cultured for 1 week with peptide pools of either HPV16 E6, HPV16 E7, HPV18 E6 or HPV18 E7. The cultured cells were tested in ELISPOT assays against the stimulating peptide pools using autologous PBMC as antigen-presenting cells. Mean spot counts from triplicate wells are shown. Only counts that were significantly greater (>2SD) than background counts and >20 spots are shown.