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. 2019 Jul 9;105(4):987–9998. doi: 10.3324/haematol.2018.214379

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Figure 4. — The mesenchymal stromal cells (MSC)-induced Side Population (SP) functionality in leukemia blasts is associated with quiescence and chemotherapy efflux through ABC transporter activation. (A) Cytograms and histograms showing gating strategies and percentage of SP and MP blasts in G0 and in G1-S-G2-M after a 3-day co-culture on HD MSC. Cell cycle status of SP versus Main Population (MP) blasts was analyzed adding pyronin Y during Hoechst staining. SP blasts are mostly in G0 [median 76% (16.5%)] compared to MP [median 33.45% (18.6%)] blasts which are in G1-S-G2-M (P=0.0009, n=12, Wilcoxon test). (B) Transcriptomic analysis of SP blasts compared to MP blasts. (C) Cytograms showing gating strategy to evaluate mitoxantrone efflux in SP or MP blasts. Quantification of mitoxantrone MFI (D) in SP [median 1,584 (1,845)] and in MP [median 3219 (1,706)] blasts (P=0.0052, n=12, Wilcoxon test). (E) DioC2,3 MFI evaluating ABCB1 activity in SP [median 239 (4,056)] and MP [median 1,298 (5418)] cells (P=0.023, n=7, Wilcoxon test). **P<0.01; ***P<0.001.