Figure 3.

Overview of the MYC gene insertion into the immunoglobulin heavy chain locus. (A) Verification of the breakpoint junctions by Sanger sequencing of the MYC gene locus insertion within the immunoglobulin heavy chain (IGH) locus in the lymph node (LN) as well as bone marrow (BM) tumor biopsy. The sequences confirmed the insertion of an 8,643 bp long sequence from chr8:128,750,275-128,758,918 bp, which was inserted between chr14:106,177,155-106,177,167 bp deleting 11 bp between the breakpoints. Moreover, at the second breakpoint five nucleotides were introduced. (B) Schematic outline of the MYC insertion into the IGH locus. Based on whole genome sequencing (WGS) data we could show that part of the MYC gene, which carries the coding exons 2 and 3, is gained and inserted IGHA1 the IGH locus within the switch region of IGHA1 (IGHA1 switch region chr14:106,715,033-106,178,629bp, hg19, Hübschmann et al., unpublished).