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. 2019 Aug 29;105(4):925–936. doi: 10.3324/haematol.2018.210286

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Figure 5. — STAT1 expression rescues the erythroid differentiation phenotype by arhgef12 knockdown in zebrafish. (A) O-Dianisidine staining results of embryos injected with control morpholino (MO) (a), arhgef12a & arhgef12b MO (b), and arhgef12a & arhgef12b MO with stat1a mRNA (c) at 4dpf. Yolk sac ventral views of the indicated microinjected embryos (a’-c’). Whole-mount in situ hybridization (WISH) results of ae1-globin in the embryos injected with control MO (e), arhgef12a & arhgef12b MO (f), and arhgef12a and arhgef12b MO with stat1a mRNA (g) at 4dpf and the corresponding magnifications of CHT (e’-g’). (B) Wright-Giemsa results of erythrocytes, which were isolated from control MO (a), arhgef12a and arhgef12b MO (b), and arhgef12a & arhgef12b MO with stat1a mRNA (c) injected embryos at 4 dpf. (C) WISH results of gata1 in the embryos injected with control MO (a), stat1a MO (b) at 4dpf and the corresponding magnifications of CHT (a’,b’). WISH results of αe1-globin in the embryos injected with control MO (c), stat1a MO (d) at 4dpf and the corresponding magnifications of CHT (c’,d’).