Figure 1.

HIF-1α is over-expressed and more active in primary cells isolated from patients with chronic lymphocytic leukemia (CLL) and in lymphoma cell lines carrying a TP53 disruption. The expression of HIF-1α and HIF-1α target genes was measured in TP53^dis and TP53^wt CLL cells and in lymphoma TP53^wt and TP53^ko cell lines. (A) Western blot (WB) analysis for HIF-1α protein expression in freshly isolated TP53^dis and TP53^wt CLL cells. A representative blot is shown with relative Unique Patient Numbers (UPN) and cumulative band intensity data obtained from the analysis of 17 TP53^wt and 15 TP53^dis CLL patients. Box plots represent median value and 25-75% percentiles, whiskers represent minimum and maximum values of band intensity for each group. (B-D) Real-time-polymerase chain reaction (RT-PCR) analysis of HIF-1A, and its target genes GLUT1 and ENO1 expression levels in TP53^dis and TP53^wt CLL cells. (E) WB analysis for HIF-1α protein expression in the TP53^wt and TP53^ko Granta-519 and Séraphine cell lines. Representative blot of three independent experiments is shown. (F) RT-PCR analysis of HIF-1A in the TP53^wt and TP53^ko Granta-519 and Séraphine cell lines. (G) RT-PCR analysis of VEGF, GLUT1 and ENO1 in the TP53^wt and TP53^ko Granta-519 and Séraphine cell lines showed a significantly higher expression level of all analyzed genes in TP53^ko samples.(B, C and D) Box and whiskers plots represent median values and 25-75% percentiles; whiskers represent minimum and maximum values for each group. (F and G) Bar graphs represent mean results obtained from three experiments together with standard error of mean. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001.