Table 3.
Elution profiles of the wild type and site-directed mutants for SARS-CoV 3CLpro in solution by SEC analyses.
| Protein | Elution peakDimer (ml) | Elution peak monomer (ml) | Dimer (%)a | Monomer (%)a | D (%)/M (%) |
|---|---|---|---|---|---|
| WT SARS-CoV 3CLpro | 44.6 | 62.1 | 50.5 | 49.5 | 1.02 |
| SARS-CoV 3CLpro Ser1Ala | 43.4 | 62.8 | 51.9 | 48.1 | 1.08 |
| SARS-CoV 3CLpro Phe3Ala | 44.8 | 63.4 | 48.2 | 51.8 | 0.93 |
| SARS-CoV 3CLpro Arg4Ala | 44.8 | 60.4 | 31.1 | 68.9 | 0.45 |
| SARS-CoV 3CLpro Ser10Ala | 45.8 | 60.2 | 39.8 | 60.2 | 0.66 |
| SARS-CoV 3CLpro Glu14Ala | 44.4 | 62.4 | 26.5 | 73.5 | 0.36 |
| SARS-CoV 3CLpro Ser139Ala | 43.8 | 59.2 | 44.8 | 55.2 | 0.81 |
| SARS-CoV 3CLpro Phe140Ala | 44.2 | 60.6 | 38.7 | 61.3 | 0.63 |
aThe percentage of dimers (D) and monomers (M) was estimated by deconvolution of the corresponding SEC elution profiles.