Table 2.
Virus concentration for incubation of embryos (TCID50/ml) | Embryo transfer with unwashed embryos | Embryo transfer with washed embryos | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Total no. of pups born (no. of litters) | Seropositive mice days post embryo transfer* | Total no. of pups born (no. of litters) | Seropositive mice days post embryo transfer* | |||||||||||||
14 | 21 | 28 | 42 | 42 | 63 | 63 | 14 | 21 | 28 | 42 | 42 | 63 | 63 | |||
R | R | R | R | P | R | P | R | R | R | R | P | R | P | |||
106 | 0 (0)† | 3 | 3 | 3 | 3 | NA | 3 | NA | 0 (0)† | 3 | 3 | 3 | 3 | NA | 3 | NA |
105 | 8 (2)† | 2 | 3 | 3 | 3 | 8 | 3 | 0 | 2 (1)† | 2 | 3 | 3 | 3 | 2 | 3 | 0 |
104 | 2 (1)‡ | 3 | 3 | 3 | 3 | 0 | 3 | 0 | 7 (1)‡ | 1 | 1 | 1 | 1 | 0 | 1 | 0 |
103 | 18 (2)† | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 14 (2)† | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
102 | 1 (1)† | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 24 (3)† | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
101 | 21 (2)† | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 25 (3)† | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Control (KSOM) | 33 (3)† | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 16 (2)† | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R, Recipients; P, progeny; NA, not available.
Three recipients were used.
Four recipients were used.