Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 16;162(5):357–369. doi: 10.1093/jb/mvx039

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Authors 2017. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved

This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.

PMC Copyright notice

Fig. 5 — Effects of the addition of Gam protein on protein synthesis activity with a linear DNA template. (A) Micro-dialysis reaction (30 µl) containing 120 ng of linear DNA template was performed with 1 ml of feed solution at 25 °C with shaking at 240 rpm (IKA KS130 basic orbital shaker). Purified Gam protein was added at a concentration of 0 (diamonds), 1 (squares), 10 (triangles), or 20 ng/µl (circles). (B) The amount of linear DNA template remaining in the reaction solution was determined by means of quantitative PCR. The linear template of GFPS2 was generated by means of PCR by using the universal U2 primer (52). The first measurement was recorded at 5 min. Each result represents the average of at least three independent experiments.