Figure 2.
Anti–virulence (V) antigen antibodies in serum evoked by AdsecV after intramuscular immunization of mice. Antibody levels were quantified by ELISA. A Dose-dependent induction of anti–V antigen IgG 4 weeks after immunization with AdsecV (n=10 mice/group). B Time course of induction of anti–V antigen IgG after immunization with AdsecV (109 particle units [pu]) or the control vector AdNull (109 pu). Serum samples were collected before and 1, 2, 4, 6, and 8 weeks after immunization (n=5 mice/group). C Anti–V antigen IgG subtypes at 5 weeks in pooled serum samples (n=10 mice/group). Data are mean±SE values except for those in panel C, in which data are from pooled serum samples. For all panels, black triangles indicate AdsecV-immunized mice, white circles indicate AdNull-immunized mice, and black diamonds indicate naive mice; dashed lines indicate the limit of detection of the assay. Data are representative of results from 2 independent experiments