Figure 4.

Virulence (V) antigen-stimulated cytokine production by CD8⁺ T cells from mice immunized with AdsecV. Mice were immunized intramuscularly with either saline (naive mice), the control vector AdNull (10¹¹ particle units [pu]), or AdsecV (10¹¹ pu). Six days after immunization, CD8⁺ T cells were isolated from spleens and stimulated for 36 h with syngeneic dendritic cells (DCs) pulsed with 100 μg/mL purified V antigen. Cytokine expression was assessed by enzyme-linked immunospot assay. Panel A shows interleukin (IL)–2 expression and panel B shows interferon (IFN)–γ expression, both for stimulation with DCs alone and for stimulation with DCs plus V antigen. Data are mean±SE values (n=5 mice/group) and are representative of results from 3 independent experiments. *P<.005, compared with the 2 control groups (analysis of variance followed by Fisher’s protected least significant difference test)