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. 2013 May;91(5):2141–2150. doi: 10.2527/jas.2012-6053

Table 1.

Primer sequences for quantitative real-time PCR

Target gene Sense (5'- 3') Antisense (5'- 3')
Acyloxyacyl hydrolase (159 bp) TCAGGGGGACAGAAATATGG CCAGAATCACGCAGAATCAC
Lysozyme (NM_214392.2; 80 bp) CGGTGCGAGTTCGCCAGAATTC AAACACACCCAGTTCGCCAGGC
Intestinal alkaline phosphatase (XM_003133729; 139 bp) GTCAGAACGGAGTTCGGAAG AGGCCCATGAGGTGTGTTAC
Claudin 3 (NM_001160075; 95 bp) CATCGGCAGCAGCATTATC ACACTTTGCACTGCATCTGG
Claudin 4 (NM_001161637.1; 110 bp) AGGTGATGGGTATCGCCCTGGC CGACGTGACGATGTTGCTGCC
Occludin (NM_00163647; 115 bp) ATCCTGGGTGTGATGGTGTT ACTGGTTGCAGAGGGCATAG
β-2-Microglobulin TGGTCTTTCTACCTTCTGGTCC TGTGATGCCGGTTAGTGGTCTC