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. 2012 Aug;90(8):2784–2793. doi: 10.2527/jas.2011-4518

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Figure 3. — (A) Serum IL-1β and (B) tumor necrosis factor (TNF)-α concentrations in pigs fed control or mannan oligosaccharide (Actigen; ACT, Alltech, Inc., Nicholasville, KY) diets with or without porcine reproductive and respiratory syndrome virus (PRRSV) infection. The concentrations of IL-1β and TNF-α of infected pigs were greater than those of uninfected ones (P < 0.001). Dietary ACT increased the IL-1β concentration (P = 0.019), but tended to decrease the TNF-α concentration (P = 0.058) in pigs as compared with the control diet. There was an ACT × PRRSV interaction for IL-1β (P = 0.016), indicating that infected pigs fed the diet with ACT had a greater IL-1β concentration than those fed the diet without ACT (P = 0.006). There were also significant effects of day or interaction of day × PRRSV on IL-1β and TNF-α (P < 0.001). Values were means; pooled SEM were 1.8 and 6.6 pg/mL for IL-1β and TNF-α, respectively. A pig was an experimental unit; each treatment had 8 pigs except ICON (7 pigs, 1 pig euthanized at 18 d postinfection). CON: uninfected control-fed pigs; ACT: uninfected ACT-fed pigs; ICON: infected control-fed pigs; IACT: infected ACT-fed pigs. †P < 0.1; *P < 0.05; **P < 0.01.