Figure 4. Deletion of Islr in stromal cells suppressed tumor growth.

• A
  Schematics of generating AOM–DSS mouse colon tumor model.
• B
  Quantification of body weight changes in control (n = 9) and cKO (n = 10) mice during tumor development.
• C
  Gross images of AOM–DSS tumors in control (n = 9) and cKO (n = 10) mice.
• D
  Quantification of tumor number per mouse and tumor volume in control (n = 9) and cKO (n = 10) mice.
• E
  Histological images of colon tumors from control and cKO mice. Scale bar: 2 mm.
• F, G
  Double immunofluorescence for Cyclin D1 and β‐catenin (F), as well as Ki67 and β‐catenin (G) in colon tumors from control (n = 4) and cKO (n = 4) mice. Scale bar: 100 μm.
• H
  Quantification of percentage of Cyclin D1⁺ and Ki67⁺ cells in (F and G); n = 4.
• I
  The growth curve of HT29 CRC cells cultured in the supernatant with or without ISLR‐HA. The supernatant was collected for HT29 CRC cells 24 h after transfected with pcDNA3.1 empty vector or pcDNA3.1‐ISLR‐HA vector; n = 5 at each timepoint.

Data information: In (B, D, H, and I), data are presented as mean ± SD. **P < 0.01; ***P < 0.001 (Student's t‐test).