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. 2020 Mar 4;39(7):e103255. doi: 10.15252/embj.2019103255

Figure EV4. Stromal cell‐secreted Islr suppressed epithelial Hippo signaling and activated YAP.

Figure EV4

  1. KEGG enrichment analysis in DEGs of hISLR overexpressing HEK293FT cells.
  2. Western blotting for HA and ISLR in the supernatant from HCT116 CRC cells transfected with pcDNA3.1 or pcDNA3.1‐hISLR‐HA plasmids.
  3. Western blotting for pMST1/2, MST1, pMOB1 and MOB1, pYAP, and YAP in lysates from HCT116 CRC cells cultured in the supernatants with or without hISLR‐HA. β‐actin was used as a loading control.
  4. Western blotting for YAP in nuclear proteins isolated from NCM460 colon epithelial cells cultured in the supernatant with or without hISLR‐HA. Histone H3, a positive control for nuclear proteins. α‐Tubulin, a positive control for cytoplasmic proteins.

Source data are available online for this figure.