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. 2020 Mar 4;39(7):e103255. doi: 10.15252/embj.2019103255

Figure EV5. Deletion of Yap1 abrogated the mIslr‐mediated promoting effect of tumor growth.

Figure EV5

  1. Western blotting for Yap1 in primary intestinal epithelial cells 24 h after Yap1 siRNA treatment. β‐actin was used as a loading control. The sequences of Yap1 siRNA are in Appendix Table S5.
  2. Generating Yap1‐deficient CT26 CRC cells using Crispr/Cas9 technique. gRNA marked by green color. Protospacer adjacent motif (PAM) sequence marked by red color. G deletion is indicated by an asterisk.
  3. Western blotting for Yap1 in normal and Yap1‐deficient (Yap1 Δ) CT26 CRC cells. α‐Tubulin was used as a loading control.
  4. qRT–PCR for Ctgf, Cry61, and Fstl1 in normal and Yap1‐deficient (Yap1 Δ) CT26 CRC cells; n = 3.
  5. Western blotting for mIslr in the supernatant from CT26 CRC cells transfected with pcDNA3.1 or pcDNA3.1‐mIslr plasmids.
  6. The growth curve of CT26 CRC cells under indicated conditions; n = 5.
  7. Gross images of xenografted tumors 10 days after transplantation. Quantification of tumor weight; n = 5.
  8. qRT–PCR for Ctgf, Cry61, and Fstl1 in CT26 CRC cells at indicated conditions; n = 3.
Data information: In (D, F–H), data are presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001 (Student's t‐test).Source data are available online for this figure.