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. 2007 Nov 15;196(Suppl 2):S237–S246. doi: 10.1086/520607

Figure 3.

Figure 3

The first 20 amino acids of the DC-SIGN (dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin) cytoplasmic tail are dispensable for augmentation of Ebola virus (EBOV) glycoprotein (GP)-dependent infection. A, DC-SIGN surface expression. Surface expression of DC-SIGN and DC-SIGN variant Δ20 on B-THP cells was analyzed by fluorescence-activated cell sorting (FACS) analysis. B, Enhancement of EBOV GP-dependent entry. The indicated B-THP cell lines were inoculated with ZEBOV GP-bearing reporter viruses, and luciferase activities in cellular lysates were determined. Data are shown relative to infection of B-THP control cells and denote the mean values (±SEMs) from 3 independent experiments. A t test (2-tailed) for independent samples was used for statistical analysis. Asterisks denote values significantly different from those measured after infection of hiDC-SIGN cells (*P ⩽ .05). C, DC-SIGN internalization. B-THP cells expressing DC-SIGN and DC-SIGN variant Δ20 were incubated with anti-DC-SIGN antibodies 507 (specific for the lectin domain) and DCN 46 (specific for the neck domain) at 4°C and were shifted to 37°C for the indicated times, followed by analysis of lectin expression by FACS. Geometric mean channel fluorescence of cells maintained at 4°C during the entire experiment was set as 100%. Data denote the mean values (±SEMs) from 3 independent experiments. A t test (2-tailed) for dependent samples was used for statistical analysis. Asterisks denote values significantly different from those measured for DC-SIGN and variant ±20 at 0 min (*P ⩽ .05). hiDC-SIGN, cells sorted for high levels of DC-SIGN expression.