Figure 6. OXGR1 expressed by adrenal gland mediates the stimulatory effects of AKG on thermogenesis and lipolysis.

• A, B
  Body weight gain (A) and cumulative food intake (B) of male OXGR1 adrenal‐specific reexpression mice (OXGR1RE^AG). Male OXGR1KO mice (8 weeks old) were adrenal‐specifically injected with control HBAAV2/9‐GFP (OXGR1KO control) or HBAAV2/9‐OXGR1 (OXGR1RE^AG). Two weeks after injections, mice were switched to HFD and further divided into two groups receiving tap water or water supplemented with 2% AKG for 12 weeks (n = 8 per group).
• C, D
  Representative image of body composition (C) and fat and lean mass index (D) of male OXGR1RE^AG mice treated with AKG for 12 weeks (n = 8 per group).
• E, F
  Weight index of gWAT (E) and iWAT (F) in male OXGR1RE^AG mice treated with AKG for 12 weeks (n = 8 per group).
• G, H
  Immunoblots (G) and quantification (H) of p‐HSL and ATGL protein in gWAT of male OXGR1RE^AG mice treated with AKG for 12 weeks (n = 8 per group).
• I–L
  Oxygen consumption (I–J) and RER (K–L) of male OXGR1RE^AG mice treated with AKG for 12 weeks (n = 8 per group).

Data information: Results are presented as mean ± SEM. In (A, B), *P ≤ 0.05 by two‐way ANOVA followed by post hoc Bonferroni tests. In (D–F, H, J and L), different letters between bars indicate P ≤ 0.05 by one‐way ANOVA followed by post hoc Tukey's tests.