Figure EV1. SMCHD1 association with telomeres is increased upon TRF2 removal.

1. Telomeric DNA ChlP with antibodies against γH2AX, SMCHD1, and rabbit lgG. Representative dot blot images of precipitated DNA detected with a (CCCTAA)n or Alu probe. ChlPs were performed in HeLa cells transfected with shTRF2 or empty vector (EV) control.
2. Bar graph for quantification of γH2AX, SMCHD1, and rabbit lgG binding to telomeric or Alu DNA as assessed by ChlP. The bars represent average values from three independent experiments for telomeric DNA and two independent experiments for Alu DNA. Error bars represent the standard deviation. P‐values were calculated by unpaired two‐tailed Student's t‐test *P < 0.05.