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. 2020 Feb 21;39(7):e102668. doi: 10.15252/embj.2019102668

Figure 5. SMCHD1 is not required for genome‐wide ATM signaling.

Figure 5

  1. Western blot detection of SMCHD1, ATMpS1981, CHK2 pT68, CHK1 pS345, RPA pS33, RPA pS4/8, γH2AX, and vinculin in wild‐type or SMCHD1 KO HeLa cells γ‐irradiated with 3 Gy dose and harvested at the indicated times (1, 12, 24 h) after the treatment.
  2. Representative images for immunofluorescence (IF) detection of ATMpS1981 in wild‐type HeLa cells γ‐irradiated with a dose of 3 Gy and harvested after the indicated times. Scale bar: 5 µm.
  3. Quantification of the average number of ATMpS1981 foci per cell detected as in (B) in wild‐type and SMCHD1 KO γ‐irradiated HeLa cells. Data represent mean of three independent experiments ± SD (> 200 cells/condition/experiment) with individual average values indicated.

Source data are available online for this figure.