Figure 3. Hydrophobic interactions between CAL1 α1 and CENP‐A α2 are critical for CENP‐A/H4 binding in cells.

1. Representative fluorescence images and quantification of tethering assays. U2OS cells containing a LacO array were co‐transfected with CENP‐A‐GFP‐LacI with CAL1_WT‐V5 and also with CAL1‐V5 carrying point mutations. Scale bar: 10 μm (n = 2 experiments).
2. Representative fluorescence images and quantification of in vivo tethering assays. Drosophila Schneider S2 cells containing a LacO array were co‐transfected with CENP‐A‐GFP‐LacI with CAL1_WT‐V5 and also with CAL1‐V5 carrying point mutations. Arrows point to the LacO site. Scale bar: is 5 μm (n = 3 experiments).

Data information: In (A), data presented as mean ± SEM of 2 experiments, n ≥ 20 cells per experiment. P‐values were calculated using a Mann–Whitney test. In (B), data presented as mean ± SEM of 3 experiments, n ≥ 45 cells per experiment, P‐values were calculated using a Mann–Whitney test (****P < 0.0001).