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. 2020 Mar 5;39(7):e103234. doi: 10.15252/embj.2019103234

Figure EV4. Mode of H4 recognition by CENP‐A by CAL1.

Figure EV4

  1. Structural superposition of CENP‐A/H4 with H3/H4 (Clapier et al, 2008). CENP‐A is shown in maroon, H4 in green and H3/H4 in silver. Arrows indicate conformational changes.
  2. Structural superposition of CAL1–CENP‐A/H4 (form I) with H3/H4 (Clapier et al, 2008). CAL1 is shown in blue, CENP‐A in maroon, H4 in green and H3/H4 in silver. Arrows indicate conformational changes; dotted arrow highlights conformational changes in the loop regions.
  3. Structural superposition of hs CENP‐A/H4 (Sekulic et al, 2010) with dm CENP‐A/H4. dm CENP‐A in maroon, dm H4 in green and hs CENP‐A/H4 in silver. Dotted arrow highlights conformational changes in the loop regions.
  4. (left panel) Quantifications of Ni‐NTA pull‐down of His‐CAL11–160 WT and indicated mutants with CENP‐A101–225–H4. Bar graph shows average ratio of the band intensities between CENP‐A101–225/H4 and His‐CAL11–160 (n = 7 experiments). (right panel) Quantifications of Ni‐NTA pull‐down of His‐CAL11–160 WT with CENP‐A101–225–H4 and indicated mutants. Bar graph shows average ratio of the band intensities between CENP‐A101–225/H4 and His‐CAL11–160 (n = 4 experiments).
Data information: In (D), (left panel) data presented as mean ± SD of 7 experiments, P‐values were calculated using a Mann–Whitney test. (right panel) Data presented as mean ± SD of 4 experiments, P‐values were calculated using unpaired two‐tailed t‐test. (*P < 0.05, ***P < 0.001, ****P < 0.0001).