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. 2016 May 6;160(5):259–268. doi: 10.1093/jb/mvw032

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© The Authors 2016. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved

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Fig. 5. — In vitro nuclear transport assay using KPNA1. (a) Permeabilized cells were incubated at 37 °C for 8 min in the presence of the ternary transport complex (KPNA1-importin-β1-NLS-GFP) and observed under the microscope. (b) A buffer control, in the absence of any transport complex (KPNA1-importin-β1-NLS-GFP). (c) In the presence of aptamer 76 (5 μM), and the ternary transport complex (KPNA1-importin-β1-NLS-GFP). (d) In the presence of aptamer 72 (5 μM), and the ternary transport complex (KPNA1-importin-β1-NLS-GFP). (e) In the presence of pool RNA (5 μM) and the ternary transport complex (KPNA1-importin-β1-NLS-GFP). For details, see the Materials and Methods. Cells were fixed after 8 min incubation and images were collected.