Figure 4.

Effect of ATA on EV71 viral RNA synthesis and EV71 3D viral polymerase activity. (a) ATA reduces the level of viral RNA synthesis. Total cellular RNA (0.5 µg) extracted from infected cells was used in each RT–PCR experiment, and 3 µL of reaction solution was added to each well. (b) ATA inhibits the activity of EV71 3D RdRp. RNA polymerase assays were performed using various concentrations of ATA as described in the Materials and methods section. Data are represented as means ± SEM from three independent experiments. The difference among different groups was assessed by one-way analysis of variance followed by post-hoc Tukey's multiple comparison test (*P < 0.05, **P < 0.001 versus the wild-type control group polymerase without inhibitor).