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. 2008 May 1;282(1):15–21. doi: 10.1111/j.1574-6968.2008.01074.x

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© 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved

This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.

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Comparative sensitivities of visual inspection and electrophoretic analyses of LAMP and PCR for the detection of Alexandrium minutum. (a) The number above each lane represents the dilution of the purified A. minutum DNA: lane M, 2-kb ladder used as a size marker; lanes 1-6, DNA of A. minutum at 1, 100, 10, 1 pg per tube, 100 and 10 fg per tube, respectively. The lower figures are electrophoretic data from the PCR analysis. PCR shows a 176-bp amplification product. (b) Lane M, 2-kb ladder used as a size marker; lanes 1–6, dilution of the A. minutum cells (DNA extracted by boiling) at 500, 100, 50, 10, 5 and 1 cells/tube, respectively. The right figure is the visual inspection of the LAMP products following the addition of SYBR Green I. The lower figure is the sensitivity of PCR for the detection of A. minutum cells as observed by agarose gel analysis.