1.
Primer | Primer type | Length | Sequence (5′→3′) |
FIP | Forward inner (5′F1C-TTTT-F2-3′) | 48 nt F1C, 25 nt; F2, 19 nt | ACMTTCTTCCAACAGCATCTCTTACTTTTGCTGTATCGTATCGTCCTG |
BIP | Backward inner (5′B1C-TTTT-B2-3′) | 48 nt B1C, 24 nt; B2, 20 nt | TCGAACAGAACAAGGTTGATTACCTTTTGGAYATTCAGATCATTCGCG |
F3 | Forward outer | 22 nt | CACCRGATACCAACCTCACAGG |
B3 | Backward outer | 23 nt | CAAGCAHACCTTCAAGMATATCC |
M, A or C; Y, T or C; H, A or C or T.
See Galluzzi et al. (2004) for sequence details.