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. 2009 Jun 18;30(8):1336–1344. doi: 10.1093/carcin/bgp150

Table I.

IRK-1 and -2 bind specifically to multiple mucosal HPV promoters

Sequence a Consensus match Cell complex Kd (nM) b rIRF-1 Kd (nM) c rIRF-2 Kd (nM) d
a HPV-16 (30-66) wt 11/13 2 2 2
b HPV-16 IRE mut I 10 nd nd
c HPV-16 IRE mut II 10 nd nd
d HPV-6 (30-66) 10/13 10 10 10
e HPV-6 (7851-7885) 12/13 3 1 1
f HPV-11 (30-66) wt 10/13 10 10 10
g HPV-11 (30-66) mut 30 nd nd
h HPV-11 (7874-7917) wt 11/13 3 1 1
i HPV-11 (7874-7917) mut 10 nd nd
j HPV-13 (32-68) 9/13 10 10 10
k HPV-31 (32-68) 10/13 3 1 1
l HPV-33 (34-70) 10/13 3 1 3
m HPV-35 (16-47) 9/13 3 nd nd
n HPV-52 (37-71) 11/13 3 10 10
o HPV-56 (42-73) 9/13 10 nd 10
p HPV-72 (24-57) 11/13 20 nd 20
q IRF consensus 13/13 nd nd nd
r ISRE (ISG15) wt 11/13 0.6 0.3 0.3
s ISRE (ISG15) mut 10 nd nd

nd, not determined.

a

Mobility shift assays were performed with synthetic oligonucleotides (as shown in Figure 1A ) and purified HaCaT nuclear extract and in vitro translated recombinant IRF-1 and IRF-2 proteins and derived from 10 mucosal HPV promoters that matched a 13 nt IRF consensus motif (IRE).

b

Dissociation constants for keratinocyte extracts were determined from an average of two to four experiments as measured by scanning densitometry.

c

Dissociation constants for rIRF-1 were determined from an average of two to four experiments as measured by scanning densitometry.

d

Dissociation constants for rIRF-2 were determined from an average of two to four experiments as measured by scanning densitometry.