Figure 3.

Increase in CCL2, CCL3, CCL5, and CXCL10 expression in H5N1/97-infected adult and neonatal monocyte-derived macrophages (MDMs), compared with that in H1N1/98-infected MDMs. After influenza virus infection, total RNA was harvested at the 7-h point of infection. The target genes were quantified by quantitative reverse-transcription polymerase chain reaction and normalized to 10⁴ copies of β-actin mRNA. The fold increase is the normalized no. of target-gene copies in H5N1/97-infected MDMs divided by the no. in H1N1/98-infected MDMs. Significantly higher fold increases in CCL3 expression were detected in H5N1/97-infected adult MDMs than in neonatal MDMs. The data shown are the mean±SE from 6 sets of independent experiments. *P<.05