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. 2010 Aug 14;12(14):1163–1169. doi: 10.1016/j.micinf.2010.08.002

Fig. 3.

Fig. 3

Immunoglobulin (Ig) expression in the brains collected from systemically perfused mice (a). Heavy (upper arrow head) and light (lower arrow head) chains of Ig were detected only in the brain of IC immunized mice (a, lane3). These Ig fraction from IC immunized mice showed significant focus reduction in virus infected mouse neuroblastoma cell (NA) (b). Whole brain lysates from virus inoculated mice also showed high Ig induction in IC immunized mice (c, lanes 2 and 5). Middle panel also demonstrates proliferated viral nucleoprotein (N) in control mice at 3 and 7 days post virus inoculation (dpi) (lanes 1 and 4) and in SC immunized mice at 7 dpi (lane 6). Vaccine (−) indicates PBS inoculated mice (control group). Tubulin was equally detected in all lanes.