Table 2.

SAAV in suckling mice

ID	Inoculated with	Outcome	Isolation of replicating virusa	Antibody titer
				IgGb	Neut.c
2:1	Inactivatedd	S	ND	−	−
2:2	Inactivated	S	ND	−	−
2:3	Inactivated	S	ND	−	−
2:4	Inactivated	S	ND	−	−
2:5	Inactivated	S	ND	−	−
2:6	Inactivated	S	ND	−	−
2:7	Inactivated	S	ND	−	−
2:8	Inactivated	S	ND	−	−
6:1	5000 FFU	S	+ (1 × 103)	2430	80
6:2	5000 FFU	S	+ (1 × 103)	270	40
6:3	5000 FFU	S	−	810	−
6:4	5000 FFU	S	−	810	20
6:5	5000 FFU	S	− (+ in PCR)	270	40
6:6	5000 FFU	S	− (+ in PCR)	90	40
6:7	5000 FFU	S	+ (0.5 × 103)	90	40
6:8	5000 FFU	S	− (+ in PCR)	270	−
7:1	500 FFU	S	− (+ in PCR)	810	40
7:2	500 FFU	S	− (+ in PCR)	810	40
7:3	500 FFU	S	− (+ in PCR)	810	40
7:4	500 FFU	S	− (+ in PCR)	270	−
7:5	500 FFU	S	− (+ in PCR)	810	80
7:6	500 FFU	S	+ (5 × 103)	810	40
7:7	500 FFU	S	+ (0.5 × 103)	270	20
7:8	500 FFU	S	−	810	20
8:1	50 FFU	S	+ (1 × 103)	810	160
8:2	50 FFU	S	−	270	20
8:3	50 FFU	S	− (+ in PCR)	810	−
8:4	50 FFU	S	−	270	40
8:5	50 FFU	S	−	270	80
8:6	50 FFU	S	−	90	−
8:7	50 FFU	S	−	270	−

S, survived, ND, not determined.

^aVirus from brains was titrated on Vero E6 cells, FFU/brain in brackets, + in PCR, negative for replicating virus, but positive for RT-PCR on DOBV/SAAV S, −, negative for replicating virus and RT-PCR.

^bReciprocal anti-DOBV N protein-IgG titer. −, Negative at 10× dilution of sample.

^cNeutralizing antibody titer. −, Negative at 20× dilution of sample.

^dHeat-inactivated SAAV, corresponding to 5000 FFU, was administered.