Fig. 1.

Kinetics of the appearance of CPE in Vero E6 cells after SARS-CoV infection. Monolayers of Vero E6 cells in 12-well microplates were infected with an MOI of 1 for 1 h at 37 °C. The cells were then washed with PBS and cultured in complete medium for 6 dpi at 37 °C. The culture medium was replaced with fresh complete medium on day 3 post-infection. The adherent viable cell numbers were counted daily (A). Then, the cell morphologies were observed daily under a light microscope (B).