Fig. 5.

Expression of SARS-CoV antigens in the persistently infected cell clones. The Vero E6 cells that were mock-infected, acutely SARS-CoV-infected as in Fig. 1, persistently SARS-CoV-infected (Vero/SARS-CoV) for 5 months, and several representative cell clones from Vero/SARS-CoV (#21 as a virus producer; #13, #18, and #34 as cell clones that had shifted from positive to negative, as shown by RT-PCR [see Fig. 4]; and #12 as an RT-PCR-negative clone already even just after cell cloning), were similarly seeded on eight-chamber glass slides. The next day, the cells were rinsed with PBS, dried, fixed with cold acetone, and then stained with anti-SARS-CoV murine MAb (3A2).