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. 2020 Mar 25;8:225. doi: 10.3389/fbioe.2020.00225

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Copyright © 2020 Maruyama, Bilesky-José, de Lima and Fraceto.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Size distributions of wet and dry calcium alginate microparticles synthesized by ionic gelation, with or without T. harzianum and scanning electron micrographs of the dry calcium alginate microparticles, with and without encapsulated T. harzianum. (A) Wet calcium alginate microparticles; (B) wet calcium alginate/fungus microparticles (1:1 w:w); (C) dry calcium alginate microparticles; (D) dry calcium alginate/fungus microparticles (1:1 w:w). The size distribution was determined using ImageJ software. The wet and dry microparticles presented mean sizes of 2000 and 800 μm, respectively. (E) Calcium alginate microparticles; (F) calcium alginate:fungus microparticles (1:1 w:w). The interiors of the microparticles with and without the fungus showed differences, due to the presence of the fungus (spores) in the second image. The images were acquired at magnifications of 95× (insert) and 250×.