Figure 3.

PD-1 was highly expressed on CXCR5⁺CD8⁺ T cells and negatively associated with disease progression. (A) Representative expression of PD-1 on CXCR5-CD8⁺ and CXCR5⁺CD8⁺ T cells from one DENV2 patient; (B) MFI of PD-1 in CXCR5⁺CD8⁺ and CXCR5-CD8⁺ T cells in DENV2 patients; (C) Representative flow cytometric dot plot of PD-1⁺CXCR5⁺ gated on CD3⁺CD8⁺ T cells from one HI and one DENV2 patient at different days after diagnosis; (D) The frequency (left) and number (right) of PD-1⁺CXCR5⁺CD8⁺ T cells in DENV2 patients at different days after diagnosis; (E) Comparison of the frequency (left) and number (right) of PD-1⁺CXCR5⁺CD8⁺ T cells in DENV2 patients with or without kidney injury; (F) Comparison of the frequency (left) and number (right) of PD-1⁺CXCR5⁺CD8⁺ T cells in two groups categorized by platelet counts; (G) Comparison of the frequency (left) and number (right) of PD-1⁺CXCR5⁺CD8⁺ T cells in DENV2 patients with or without liver injury; (H) Comparison of the frequency (left) and number (right) of PD-1⁺CXCR5⁺CD8⁺ T cells in DENV2 patients with or without myocardial injury. KI: kidney injury; LI: liver injury; MI: myocardial injury; PLT: platelets.