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. 2005 Jul 14;10(1):32–37. doi: 10.1016/j.ijid.2005.02.003

Figure 2.

Figure 2

The dynamic range of the assay was tested running 10-fold serial dilutions of the authors’ cRNA standard ranging from 10 to 1012 molecules in the reaction. The amplification plot shows delta Rn on the y-axis (Rn: fluorescence emission intensity of the reporter dye normalized to a passive reference, delta Rn: Rn of an unreacted sample – Rn value of the reaction) against the cycle number displayed on the x-axis. The dynamic range of the assay spans 6 logs ranging from 102 to 108 log molecules of SARS-CoV RNA per reaction, corresponding to CT values ranging from 37.45 ± 0.04 for 102 copies to 17.40 ± 0.04 for 108 copies. cRNA standards with a copy number above or below the dynamic range are not shown.