Fig. 1.

AS-IV inhibits cervical cancer cells invasion and xenograft tumor growth in xenograft models. a CCK-8 assay detected proliferation of SiHa and HeLa cells treated with different concentrations of AS-IV for 72 h. b The xenograft model of cervical cancer was generated by injecting SiHa cell lines into BALB/c nude mice. AS-IV (a dose of 12.5 mg/kg/d, 25 mg/kg/d, 50 mg.kg/d) was applied for tumor rescue in mice for 35 days by gastric gavage and the inhibition of tumor growth by AS-IV was evaluated. c HeLa and SiHa cell invasion was examined by transwell analysis in the present of AS-IV of different concentrations treated for 12 h. d Expression of LC3I/II in HeLa and SiHa cells was measured by western blot in the presence of AS-IV of different concentrations treated for 12 h. Data are presented as mean ± standard deviation, n = 3. *P < 0.05 and **P < 0.01 refers to the 0 μM AS-IV control group