Fig. 5.

AS-IV induces autophagy and suppresses cervical cancer invasion by targeting DCP1A and TMSB4X. a Differentially expressed protein quantification by mass spectrometry-based targeted proteomics (PRM). b Expression of Atg7 and Atg12 in SiHa and HeLa cells was measured by western blot in the presence of AS-IV of different concentrations treated for 12 h. c SiHa and HeLa cells were treated with AS-IV in the presence of DCP1A siRNA or TMSB4X siRNA for 12 h and cell invasion was measured by transwell chamber culture. d SiHa and HeLa cells were cultured with AS-IV in the presence of DCP1A siRNA or TMSB4X siRNA for 12 h, and the expression of Atg7, Atg12, and LC3I/II was measured by western blot. e Mechanism map of AS-IV inducing autophagy through the TMSB4X/Akt/Atg5/Atg12 and DCP1A/WDFY3/Atg12 pathways. Results are presented as mean ± standard deviation, n = 3. *P < 0.05 and **P < 0.01 in comparison to the control group (0 μM AS-IV or AS-IV treated group)