Table 1.
Comparison of Pneumo4B multiplex qPCR and bacterial culture for Mannheimia haemolytica, Pasteurella multocida, Histophilus somni in 92 tracheal aspirate (TA) samples.
| n (%) TA samples | ||||||||
|---|---|---|---|---|---|---|---|---|
| Pathogen | C + PCR+ | C + PCR- | C-PCR+ | C- PCR- | Diagnostic sensitivity | Diagnostic specificity | Cohen’s kappa coefficient | |
| M. haemolytica | 18 (19.5) | 7 (7.6) | 6 (6.5) | 61 (66.3) | 0.72 | 0.91 | 0.64 | |
| P. multocida | 23 (25.0) | 4 (4.3) | 8 (8.7) | 57 (61.9) | 0.85 | 0.88 | 0.69 | |
| H. somni | 23 (25.0) | 4 (4.3) | 2 (2.1) | 63 (68.4) | 0.85 | 0.96 | 0.84 | |
C+, culture positive; C-, culture negative; PCR+, Pneumo4B positive; PCR-, Pneumo4B negative.