Table 2.
Comparison of Pneumo4B multiplex qPCR for Mycoplasma bovis and Pneumo4 V multiplex RT-qPCR and reference (reference standard) PCR methods (R) for detection of bovine parainfluenza virus type 3 (BPI3), bovine corona virus (BCoV) and bovine respiratory syncytial virus (BRSV) in 84 tracheal aspirate (TA) samples.
| No. (%) of TA samples | |||||||
|---|---|---|---|---|---|---|---|
| Pathogen | R + PCR+ | R + PCR- | R-PCR+ | R- PCR- | Diagnostic sensitivity | Diagnostic specificity | Cohen’s kappa coefficient |
| M. bovis | 25 (29.7) | 1 (1.2) | 17 (20.2) | 41 (48.8) | 0.96 | 0.71 | 0.57 |
| BPI3 | 4 (4.7) | 0 (0) | 3 (3.5) | 77 (91.6) | 1.0 | 0.96 | 0.71 |
| BCoV | 19 (22.6) | 0 (0) | 3 (3.5) | 62 (73.8) | 1.0 | 0.90 | |
| BRSV | 11 (13.0) | 1 (1.1) | 3 (3.5) | 69 (82.4) | 0.92 | 0.96 | 0.82 |
R+, reference PCR positive; R-, reference PCR negative; PCR+, Pneumo4 V/4B positive; PCR-, Pneumo4 V/4B negative.