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. 2020 Mar 31;11:141. doi: 10.1186/s13287-020-01645-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Morphology of differentiated hASCs under invert microscope. hASCs with typical cobblestone morphology similar to HaCaT cells were considered differentiated cells (white arrow). I 2D differentiation ((A) hASCs indirectly co-cultured with HaCaT cells at ALI; (B) hASCs indirectly co-cultured with HaCaT cells under ALI; (C) hASCs cultured under ALI without HaCaT’s co-cultivation). II 3D differentiation. III Controls ((NC) undifferentiated hASCs as negative control, (PC) HaCaT cells as positive control). IV Quantification of differentiation rate (%) at different time points (**p < 0.01, ***p < 0.001)