Table 1.

Overview of published studies on the role of PI4KIIIα and β in the HCV life cycle.

Authors	Year	PI4KIII isoform	HCV genotype	Methods and results
Trotard et al.	2009	PI4KIIIα and β	Replicon 1a, 1b	siRNA library screen.
			HCVpp 1a, 1b, 2a	Genotype dependency:
			HCVcc 2a	- Replication: PI4KIIIα required for 1a, 2a, PI4KIIIβ for 1a
				- Entry: PI4KIIIα required for 1a, PI4KIIIβ for 1a, 1b.
Berger et al.	2009	PI4KIIIα	Replicon 1b, 2a	siRNA library screen, validation by individual siRNAs and microscopy.
			HCVcc 2a	PI4KIIIα is required for HCV replication but not for HCV entry.
Borawski et al.	2009	PI4KIIIα and β	Replicon 1a, 1b	siRNA library screen.
			HCVcc 2a	Genotype dependency:
				PI4KIIIα: 1a, 1b and 2a; PI4KIIIβ: 1a and 1b.
Tai et al.	2009	PI4KIIIα	Replicon 1b	siRNA library screen, validation by individual siRNAs and microscopy.
			HCVcc 2a	PI4KIIIα is required for HCV replication.
Vaillancourt et al.	2009	PI4KIIIα	Replicon 1a, 1b, 2a	shRNA library screen, validation by siRNA.
				HCV replication is inhibited by PI4KIIIα silencing (genotype independent).
Hsu et al.	2010	PI4KIIIβ	Replicon 2a	siRNA knockdown of PI4KIIIα/β.
				Expression of a kinase-dead PI4KIIIβ inhibited HCV replication.
Reiss et al.	2011	PI4KIIIα	Replicon 1b, 2a	siRNA library screen, validation by individual siRNAs and microscopy. NS5A recruits and activates PI4KIIIα.
			HCVcc 2a
Berger et al.	2011	PI4KIIIα	HCVcc 2a	siRNA trans-complementation assay, microscopy, coimmunoprecipitation.
				NS5A enhances PI4KIIIα activity.
Lim et al.	2011	PI4KIIIα	Replicon 1b	Coimmunoprecipitation (NS5A interacts with PI4KIIIα), siRNA knockdown.
			HCVcc 2a
Tai et al.	2011	PI4KIIIα and β	HCVcc 2a	shRNA knockdown, microscopy.
				PI4KIIIβ silencing inhibits HCV infection but does not disturb HCV membranous web formation.
Coller et al.	2012	PI4KIIIβ	HCVcc 2a	RNAi analysis of host factors combined with live cell imaging of HCV core trafficking.
				PI4KIIIβ seems essential for HCV secretion, not for HCV replication.
Zhang et al.	2012	PI4KIIIβ	HCVcc 2a	siRNA knockdown of PI4KIIIβ, Sac1 expression and microscopy.
				PI4KIIIβ is required for HCV replication.
Bianco et al.	2012	PI4KIIIα	Replicon 1b, 2a	AL-9, a specific inhibitor of PI4KIIIα, inhibits HCV replication.
			HCVcc 2a