Fig. 1.

(A) Diagram of TransFactor inflammation profiling kit method. Cell extracts are added to 96-well plates coated with the wildtype DNA elements of individual transcription factors. Bound protein samples are then detected and quantified by primary and secondary antibody incubation. (B) Diagram of the PathDetect method for in vivo signal transduction assessment. The luciferase reporter gene is driven by a basic TATA promoter and an inducible enhancer bound proteins which recognize the AP-1 specific enhancer element are detected by an increase in reporter gene transcription.