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. 2020 Mar 23;26:e920793-1–e920793-9. doi: 10.12659/MSM.920793

Figure 2.

Figure 2

Upregulation of lncRNA MEG3 promoted cell apoptosis in HPMECs. Control-plasmid or lncRNA MEG3-plasmid were transfected into HPMECs for 24 hours and the transfection efficiency was evaluated by qRT-PCR. (A) qRT-PCR analysis of the level of lncRNA MEG3 in HPMECs after transfection. (B) Effect of lncRNA MEG3-plasmid on HPMECs apoptosis was detected by the flow cytometry assay. (C) Percentages of apoptotic cells were quantified. (D) Influence of lncRNA MEG3-plasmid on HPMEC caspase-3 activity was determined by the caspase-3 assay kit. * P<0.05; ** P<0.01. lncRNA – long noncoding RNA; MEG3 – maternally expressed gene 3; HPMEC – human pulmonary microvascular endothelial cell; qRT-PCR – quantitative reverse transcription PCR.