Fig. 2.

Stability of the E2/E2-p7 monomers in the presence of DTT. MDBK cells were infected with BVDV at MOI 1. Eighteen hours pi cells were pulse-labeled with [³⁵S]methionine/cysteine for 15 min and chased for 15 min in the absence of DTT. After this initial chase, 5 mM DTT was added (+) or not (−) to the cells and the incubation continued for additional 15 min (A) or for the times indicated (B). After the chase, the cells were harvested, lysed, and immunoprecipitated with anti-E2 MAb 214. Proteins bound were separated by SDS–8% PAGE under both nonreducing (nr) and reducing (r) conditions (A), or reducing conditions only (B). The black arrows show the shift of the bands corresponding to the E2/E2-p7 glycoproteins run under nonreducing and reducing conditions. The bands marked with an asterisk represent unspecific contaminating proteins.