Table 3. Photomotor response assay at lowest observed effect concentration at 24 hpf and 5dpf for all extracts.
Baseline, excitatory, and refractory LOEC were assessed at 24 hpf and all, dark, and light activity LOEC at 5 dpf, with at least 50% change from control. Hypo- or hyperactivity indicated and value in parenthesis reflect response at corresponding lowest dilution of sample of bloom (p<0.01) where x (or no dilution of the sample) contained 17.1 and 308.4 μg/L MCS in EtOH extracts 1 and 2, respectively, along with detectable Anabaenopeptins and Cyanopeptolins, and no MCs detected in the CHCl3 extracts (see Table 2).
| Extract from Rio De La Plata, Uruguay | 24 hpf PMR LOEC | 5 dpf PMR LOEC | ||||
|---|---|---|---|---|---|---|
| (type of sampling site) | Baseline | excitatory | refractory | all | dark | light |
| Rio de la Plata CHCl3 extract 1 | n/a | n/a | n/a | 0.01× (hyper) | 0.01× (hyper) | 0.01× (hyper) |
| Rio de la Plata CHCl3 extract 2 | no dilution | 0.1× (hypo) | n/a | no dil (hyper) | 0.01× (hyper) | n/a |
| Rio de la Plata EtOH extract 1 | n/a | n/a | n/a | 0.1× (hyper) | 0.1× (hyper) | 0.01× (hypo) |
| Rio de la Plata EtOH extract 2 | n/a | n/a | n/a | 0.01× (hyper) | 0.01× (hyper) | no dil (hyper) |
n/a signifies no significant perturbation of PMR
(hyperactive or hypoactive response indicated)