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. 2020 Mar 24;26:e920642-1–e920642-11. doi: 10.12659/MSM.920642

Figure 2.

Figure 2

PIK3C3 overexpression suppressed esophageal squamous cell carcinoma (ESCC) cell proliferation. (A) After 24 hours of transfection with pCMV6/PIK3C3 recombinant plasmid, the KYSE-150 and TE-12 cells subjected to detection of PIK3C3 by western blot. (B) MTT assay was conducted to analyze the cell viability of PIK3C3 overexpressed KYSE-150 and TE-12 cells, * P<0.05. (C) Colony formation experiment was carried out to estimate the influences of PIK3C3 overexpression on ESCC cell proliferation, * P<0.05. (D) Flow cytometry analysis was conducted on PIK3C3 overexpressed KYSE-150 and TE-12 cells to measure the effects of PIK3C3 on ESCC cell cycle, * P<0.05. pCMV6/control transfected cell was used as negative control.