Skip to main content
. 2020 Mar 24;26:e920642-1–e920642-11. doi: 10.12659/MSM.920642

Figure 4.

Figure 4

MiR-340-5p targeted and negatively regulated PIK3C3. (A) Target miRNAs of PIK3C3 were predicted by microRNA (miRNA), miRWalk and Targetscan, and the Venn diagram showing the intersection. (B) The correlation between miR-340-5p or miR-381-3p and PIK3C3 in 162 esophageal squamous cell carcinoma (ESCC) samples. Figures were downloaded from starBase database. (C) Upper panel, the wild type and mutant sequence of predicted binding sites of miR-340-5p in the 3′-UTR of PIK3C3. Lower panel, the interplay between miR-340-5p and PIK3C3 was verified by luciferase reporter assay. (D) Protein expression of PIK3C3 was detected by western blot in miR-340-5p overexpressed and blocked KYSE-150 and TE-12 cells.